Fluorescence spectral characteristics of novel asymmetric monomethine cyanine dyes in nucleic acid solutions
نویسندگان
چکیده
منابع مشابه
“Turn-On” Protein Fluorescence: In Situ Formation of Cyanine Dyes
Protein reengineering of cellular retinoic acid binding protein II (CRABPII) has yielded a genetically addressable system, capable of binding a profluorophoric chromophore that results in fluorescent protein/chromophore complexes. These complexes exhibit far-red emission, with high quantum efficiencies and brightness and also exhibit excellent pH stability spanning the range of 2-11. In the cou...
متن کاملBenz[c,d]indolium-containing Monomethine Cyanine Dyes: Synthesis and Photophysical Properties.
Asymmetric monomethine cyanines have been extensively used as probes for nucleic acids among other biological systems. Herein we report the synthesis of seven monomethine cyanine dyes that have been successfully prepared with various heterocyclic moieties such as quinoline, benzoxazole, benzothiazole, dimethyl indole, and benz[e]indole adjoining benz[c,d]indol-1-ium, which was found to directly...
متن کاملAbnormal fluorescence behavior of some cyanine dyes in a cyanine dye–TiO colloids system
The absorption and fluorescence spectra of five typical cyanine dyes adsorbed on the surface of TiO colloids have been 2 studied. The absorption spectra show that there are two kinds of isomer produced by intramolecular torsional motion of these dyes in their ground states. The fluorescence quantum yields of these dyes may be enhanced or reduced in the presence of TiO colloids. This behavior ca...
متن کاملPii: S0143-7208(98)00046-1
By condensation of quaternary benzothiazolium, quinolinium and acridinium salts having an active methyl group with 1-(3-bromopropyl)-4-chloroquinolinium or 1-(3-bromo-2-hydroxypropyl)-4-chloroquinolinium salts in the presence of a basic agent such as triethylamine, 8 asymmetric and symmetric monomethine cyanine dyes bearing !-bromopropyl substituent with one or two positive charges, were synthe...
متن کاملSequence-Dependent Fluorescence of Cyanine Dyes on Microarrays
Cy3 and Cy5 are among the most commonly used oligonucleotide labeling molecules. Studies of nucleic acid structure and dynamics use these dyes, and they are ubiquitous in microarray experiments. They are sensitive to their environment and have higher quantum yield when bound to DNA. The fluorescent intensity of terminal cyanine dyes is also known to be significantly dependent on the base sequen...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEBS Letters
سال: 1997
ISSN: 0014-5793
DOI: 10.1016/s0014-5793(97)00171-3